Journal
NUCLEIC ACIDS RESEARCH
Volume 31, Issue 15, Pages 4332-4344Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkg647
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When iron repletes, Schizosaccharomyces pombe cells repress transcription of genes encoding components involved in the reductive iron transport system. Fep1 mediates this transcriptional control by interacting specifically with GATA-type cis-acting elements. To further investigate the role that Fep1 plays in iron homeostasis, we searched for additional Fep1-regulated genes. We found that str1(+) is subject to negative transcriptional regulation, which is exerted through binding of Fep1 to a single GATA element in the str1(+) promoter. Introduction of str1(+) into a Saccharomyces cerevisiae fet3Delta arn1-4Delta strain led to assimilation of iron from ferrichrome, revealing that Str1 functions as a siderophore-iron transporter in S.pombe. We also identified two additional target genes of Fep1, named str2(+) and str3(+). We demonstrate that the str1(+), str2(+) and str3(+) genes share a common promoter element, 5'-(A/T)GATAA-3'. We found that the N-terminal 241 residue segment of Fep1 expressed in Escherichia coli specifically interacts with the 5'-(A/T)GATAA-3' element present in each of these promoters. Consistent with this, constitutive high level str1(+), str2(+) and str3(+) gene expression was observed in a fep1Delta mutant strain. Taken together, these results demonstrate that Fep1 occupies a central role in coordinating transcriptional regulation of genes encoding components of the reductive and non-reductive iron transport systems in fission yeast.
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