Journal
BIOMATERIALS
Volume 24, Issue 17, Pages 2965-2973Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0142-9612(03)00095-4
Keywords
polyacrylamide microgels; immobilization of glucose oxidase; cross-linking and glass transition; glucose biosensor
Ask authors/readers for more resources
Glucose oxidase (GO(x)) has been immobilized in cross-linked polyacrylamide microgels using the concentrated emulsion polymerization method. The immobilization of the enzyme is effective when the number of cross-links is equal or greater than 0.021 mol/g. The swelling of the microgels is not affected by the entrapped enzyme but decreases with increasing the number of cross-links. The glass transition temperature of microgels with GO(x) presents considerable differences with that of the empty microgels. which are explained on base of the competition between the plasticizer effect of the encapsulated enzyme and the chain mobility restrictions imposed by the microgel cross-linking content. Immobilization of GO(x) slightly modifies the X-ray diffraction patterns of the microgels, however, the diffraction profiles of cross-linked microgels suggest the formation of bis(acrylamide) aggregates in the outer layers of the microgel particles. (C) 2003 Elsevier Science Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available