Journal
JOURNAL OF BACTERIOLOGY
Volume 185, Issue 16, Pages 4883-4890Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.185.16.4883-4890.2003
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Funding
- NIGMS NIH HHS [GM-47446, R01 GM047446, S06 GM052588, GM-052588, GM5-59298] Funding Source: Medline
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The Bacillus subtilis extracytoplasmic function sigma factor sigma(Y) is of unknown function. We demonstrate that the sigY operon is expressed from an autoregulatory promoter site, P-Y. We selected for transposon-induced mutations that upregulate P-Y transcription in an attempt to identify genes involved in sigma(Y) regulation. The resulting insertions disrupted yxlC, the gene immediately downstream of sigY. However, the phenotype of the yxlC::Tn10 insertion was due to polarity on the downstream genes of the sigY operon; a nonpolar insertion in yxlC did not lead to derepression of P-Y. Further analyses revealed that both yxlD and yxlE encoded proteins important for the negative regulation of sigma(Y) activity. A comparison of the transcriptomes of wild-type and yxlC:: Tn10 mutant strains revealed elevated expression of several operons. However, only one additional gene, ybgB, was unambiguously identified as a direct target for sigma(Y). This was supported by analysis of direct targets for sigma(Y) transcription with whole-genome runoff transcription followed by macroarray analysis.
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