Journal
JOURNAL OF BACTERIOLOGY
Volume 185, Issue 16, Pages 5012-5014Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.185.16.5012-5014.2003
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Funding
- NIGMS NIH HHS [GM63790, R01 GM063790-01A1, R01 GM063790] Funding Source: Medline
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Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.
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