Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 474, Issue 2-3, Pages 199-204Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-2999(03)02075-2
Keywords
honokiol; magnolol; Ca2+; cortical neuron, rat; SH-SY5Y neuroblastoma
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We examined the intracellular Ca2+ response in primary cultured rat cortical neurons and human neuroblastoma SH-SY5Y cells by Fluo 3 fluorescence imaging analysis. In these two kinds of neuronal cells, honokiol and magnolol increased cytoplasmic free Ca2+ with a characteristic lag phase. The cytoplasmic free Ca2+ increase was independent of extracellular Ca2+, but dependent on activation of phospholipase C and inositol 1,4,5-triphosphate (IP3) receptors. These results suggest that honokiol and magnolol increase cytoplasmic free Ca2+ through a phospholipase C-mediated pathway, and that the release of Ca2+ from intracellular stores mainly contributes to the increase in cytoplasmic free Ca2+. Thus, honokiol and magnolol may be involved in a new activation mechanism closely associated with intracellular Ca2+ mobilization. (C) 2003 Elsevier B.V. All rights reserved.
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