Journal
FEMS MICROBIOLOGY LETTERS
Volume 225, Issue 1, Pages 53-57Publisher
OXFORD UNIV PRESS
DOI: 10.1016/S0378-1097(03)00490-7
Keywords
actinomycetes; conjugation; antibiotic; site-specific recombination
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The filamentous actinomycete Nonomuraea sp. ATCC39727 produces the industrially important glycopeptide antibiotic A40926. We developed a gene transfer system based on intergeneric conjugation from Escherichia coli. Analysis of the ex-conjugants revealed that the incoming plasmid pSET152 had integrated at two sites in the Nonomuraea genome. One of these was characterized and found to be highly related to other PhiC31 attB sites described in Streptomyces spp., including the core TTS sequence, where crossover occurs. Surprisingly, pSET152 was also found in episomic form in the Nonomuraea ex-conjugants. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
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