Journal
BIOSENSORS & BIOELECTRONICS
Volume 18, Issue 9, Pages 1125-1134Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/S0956-5663(02)00244-0
Keywords
cellulose acetate; ferricyanide; enhanced selectivity; ethanol detection; microbial biosensor; Gluconobacter oxydans
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A ferricyanide mediated microbial biosensor for ethanol detection was prepared by surface modification of a glassy carbon electrode. The selectivity of the whole Gluconobacter oxydans cell biosensor for ethanol determination was greatly enhanced by the size exclusion effect of a cellulose acetate (CA) membrane. The use of a CA membrane increased the ethanol to glucose sensitivity ratio by a factor of 58.2 and even the ethanol to glycerol sensitivity ratio by a factor of 7.5 compared with the use of a dialysis membrane. The biosensor provides rapid and sensitive detection of ethanol with a limit of detection of 0.85 muM (S/N = 3). The selectivity of the biosensor toward alcohols was better compared to previously published enzyme biosensors based on alcohol oxidase or alcohol dehydrogenases. The biosensor was successfully used in an off-line monitoring of ethanol during batch fermentation by immobilized Saccharomyces cerevisiae cells with an initial glucose concentration of 200 g l(-1). (C) 2002 Elsevier Science B.V. All rights reserved.
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