Journal
JOURNAL OF EXPERIMENTAL MEDICINE
Volume 198, Issue 4, Pages 615-621Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1084/jem.20030448
Keywords
dendritic cell; T cell priming; CCL21; imgration; dendritic cell-based vaccination
Categories
Ask authors/readers for more resources
Antigen-pulsed dendritic cells (DCs) are used as natural adjuvants for vaccination, but the factors that influence the efficacy of this treatment are poorly understood. We investigated the parameters that affect the migration of subcutaneously injected mouse-mature DCs to the draining lymph node. We found that the efficiency of DC migration varied with the number of injected DCs and that CCP7(+/+) DCs migrating to the draining lymph node, but not CCR7(-/-) DCs that failed to do so, efficiently induced a rapid increase in lymph node cellularity, which was observed before the onset of T cell proliferation. We also report that DC migration could be increased up to 10-fold by preinjection of inflammatory cytokines that increased the expression of the CCR7 ligand CCL21 in lymphatic endothelial cells. The magnitude and quality of CD(4+) T cell response was proportional to the number of antigen-carrying DCs that reached the lymph node and could be boosted up to 40-fold by preinjection of tumor necrosis factor that conditioned the tissue for increased DC migration. These results indicate that DC number and tissue inflammation are critical parameters for DC-based vaccination.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available