Journal
CHEMPHYSCHEM
Volume 4, Issue 8, Pages 793-808Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.200200565
Keywords
fluorescence spectroscopy; FRET (fluorescence resonance energy transfer); single-molecule studies
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This review presents an overview of the fluorescence detection and spectroscopy of single molecules (SMS) in the liquids and on surfaces under ambient conditions. The various techniques of SMS, such as confocal epifluorescene detection and wide-field imaging are presented and discussed together with the different methods of data analysis such as fluorescence correlation spectroscopy and burst-by-burst analysis. Selected applications of the various techniques in physics, chemistry and biology are described.
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