Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 278, Issue 34, Pages 31717-31721Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.M305448200
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The human Dnmt2 protein is one member of a protein family conserved from Schizosaccharomyces pombe and Drosophila Melanogaster to Mus musculus and Homo sapiens. It contains all of the amino acid motifs characteristic for DNA-(Cytosine-C5) methyltransferases, and its structure is very similar to prokaryotic DNA methyltransferases. Nevertheless, so far all attempts to detect catalytic activity of this protein have failed. We show here by two independent assay systems that the purified Dnmt2 protein has weak DNA methyltransferase activity. Methylation was observed a CG sites in a loose ttnCG(ga(g/a)) consensus sequence, suggesting that Dnmt2 has a more specialized role than other mammalian DNA methyltransferases.
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