Journal
FEMS MICROBIOLOGY ECOLOGY
Volume 45, Issue 3, Pages 229-238Publisher
OXFORD UNIV PRESS
DOI: 10.1016/S0168-6496(03)00154-5
Keywords
Aspergillus parasiticus; Aspergillus nomius; Aspergillus flavus; aflatoxin; sugarcane field soil; heteroduplex panel analysis; internal transcribed spacer region
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Heteroduplex panel analysis (HPA) was previously developed for genetic identification of Aspergillus Section Flavi strains, utilizing polymerase chain reaction-amplified fragments of the internal transcribed spacer (ITS) regions of the rRNA gene. Application of HPA to a field study demonstrated that a new type of FP-1 strains belonging to Section Flavi is predominantly distributed throughout sugarcane field soil in the southernmost islands of Japan, and such a trend may also be the case in Vietnam. All of the 71 tested isolates of type FP-1 were able to produce aflatoxins B and G. The morphological observations of the type FP-1 isolates showed that a major part of them had broad interfaces with Aspergillus parasiticus and the remainder with Aspergillus flavus. Phylogenetic analysis based on the ITS sequences indicated that type FP-1 formed an independent clade positioned between A. parasiticus and A. flavus, and was more closely related to the former species. This is also the first report on the distribution of Aspergillus nomius in sugarcane field soil and/or sugarcane stems in Japan and Vietnam. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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