Journal
FEBS LETTERS
Volume 550, Issue 1-3, Pages 114-118Publisher
WILEY
DOI: 10.1016/S0014-5793(03)00847-0
Keywords
protein translocation; sec machinery; SecDF-YajC; SecG; inverted membrane vesicle
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SecA and an apparatus comprising SecYEG and SecDF-YajC complexes catalyze protein translocation across the Escherichia coli membrane. SecDF-YajC and SecG facilitate membrane insertion of SecA, which is the driving force for protein translocation. Here we report that SecDF-YajC depletion together with SecG depletion nearly completely inhibits protein translocation both in vivo and in vitro, although SecDF-YajC had been thought to be unnecessary for in vitro translocation. The level of SecG in membranes decreased to about half upon SecDF-YajC depletion and recovered to a normal level when SecDF-YajC was expressed. SecDF-YajC inhibited disulfide bond formation between two SecG molecules possessing a single cysteine residue. These results suggest functional interaction between SecDF-YajC and SecG. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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