Journal
TALANTA
Volume 60, Issue 6, Pages 1223-1228Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(03)00230-3
Keywords
creatine; creatinine; amperometric biosensors; creatininase; creatinase; sorcosine oxidase
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In order to determine creatine and creatinine amperometric biosensors were proposed. A bienzymatic biosensor based on creatinase (Cl) and sarcosine oxidase (SO) was used for the assay of creatine and a trienzymatic biosensor based on Cl. SO and creatininase (CA) for the assay of creatinine. The linear concentration ranges are of pmol l(-1) to nmol l(-1) magnitude order, with very low limits of detection. The biosensors proved high reliability for determination of creatine and creatinine as raw material, and in the pharmaceutical formulation. (C) 2003 Elsevier B.V. All rights reserved.
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