Journal
SCIENCE
Volume 301, Issue 5637, Pages 1233-1235Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1085399
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In order to investigate the behavior of single molecules under conditions far from equilibrium, we have coupled a microfabricated laminar-flow mixer to a confocal optical system. This combination enables time-resolved measurement of Forster resonance energy transfer after an abrupt change in solution conditions. Observations of a small protein show the evolution of the intramolecular distance distribution as folding progresses. This technique can expose subpopulations, such as unfolded protein under conditions favoring the native structure, that would be obscured in equilibrium experiments.
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