Journal
JOURNAL OF VIROLOGY
Volume 77, Issue 18, Pages 10119-10124Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.77.18.10119-10124.2003
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A novel Alu-long terminal repeat (LTR)-based real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type I (HIV-1) DNA in infected cells with both accuracy and high sensitivity (six proviruses within 50,000 cell equivalents). Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication.
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