Journal
BIOMATERIALS
Volume 24, Issue 21, Pages 3815-3824Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0142-9612(03)00263-1
Keywords
polymers; osteoblasts; poly epsilon-caprolactone; hydroxyapatite; biodegradation
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Current methods for the replacement of skeletal tissue involve the use of autografts, allografts and, recently, synthetic substitutes, which provide a proper amount of material to repair large bone defects. Engineered bone seems a promising approach, but a number of variables have to be set prior to any clinical application. In this study. four different poly caprolactone-based polymers (PCL) were prepared and tested in vitro using osteoblast-like Saos-2 cells. Differences among three-dimensional polymers include porosity, addition of hydroxyapatite (HA) particles, and treatment with simulated body fluid. Biochemical parameters to assess cell/material interactions include viability, growth, alkaline phosphatase release, and mineralization of osteoblastic cells seeded onto three-dimensional samples, while their morphology was observed using light microscopy and SEM. Preliminary results show that the polymers, though degrading in the medium, have a positive interaction with cells, as they support cell growth and functions. In the short-term culture (3-7 days) of Saos-2 on polymers, little differences were found among PCL samples, with the presence of HA moderately improving the number of cells onto the surfaces. In the long term (3-4 weeks), it was found that the HA-added polymers obtained the best colonization by cells, and more mineral formation was observed after coating with SBF It can be concluded that PCL is a promising material for three-dimensional scaffold for bone formation. and the presence of bone-like components improves osteoblast activity. (C) 2003 Elsevier Science Ltd. All rights reserved.
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