Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 67, Issue 9, Pages 1916-1922Publisher
OXFORD UNIV PRESS
DOI: 10.1271/bbb.67.1916
Keywords
genistein; oxidative stress; nuclear factor kappa B (NF kappa B); antioxidant; macrophages
Ask authors/readers for more resources
This study was designed to investigate whether genistein may ameliorate oxidative stress and nuclear factor kappaB (NFkappaB) activation in the lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cell line. Treatment of RAW 264.7 cells with genistein significantly reduced lipopolysaccharide (LPS)-stimulated nitric oxide (NO) production in a dose-dependent manner with an IC50 of 69.4 mum. Genistein at 50 mum and 100 mum concentrations reduced thiobarbituric acid-reactive substances (TBARS) accumulation, increasing the GSH level and antioxidant enzyme activities, such as superoxide dismutase (SOD) and catalase. The specific DNA-binding activities of nuclear factor kappaB (NFkappaB) on nuclear extracts from 50 mum and 100 mum genistein treatments were significanly suppressed. These results suggest that genistein has mild antioxidant activity to suppress intracellular oxidative stress and NFkappaB activation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available