Journal
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Volume 29, Issue 3, Pages 410-418Publisher
AMER THORACIC SOC
DOI: 10.1165/rcmb.2002-0247OC
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Funding
- NHLBI NIH HHS [R01 HL67088-02] Funding Source: Medline
- NIDDK NIH HHS [P50 DK53090, P30 DK54781] Funding Source: Medline
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We investigated cystic fibrosis transmembrane conductance regulator (CFTR) activation by clinically used phosphodiesterase inhibitors (PDEis) in Calu-3 cell monolayers alone and in combination with A(2B) adenosine receptor stimulation. This receptor pathway has previously been shown to activate wild-type and mutant CFTR molecules. Several PDEis, including milrinone, cilostazol (Pletal), papaverine, rolipram, and sildenafil (Viagra), produced a short circuit current (I-sc) that was glibenclamide-sensitive, achieving 20-85% of forskolin-stimulated I-sc Papaverine, cilostazol, and rolipram also augmented both the magnitude and the duration of I-sc following low dose stimulation of adenosine receptors with Ado (0.1-1.0 muM, P < 0.01). Subsequent studies demonstrated that very low concentrations of cilostazol or papaverine (similar to 1/2 peak serum concentrations) were sufficient to activate I-sc and both agents markedly augmented Ado-stimulated (1 muM, P < 0.01). Our results provide evidence that select PDEis, at concentrations achieved as part of systemic therapies, can activate CFTR-dependent I-sc in Calu-3 cell monolayers. These studies also indicate that PDEis have the capacity to augment an endogenous CFTR-activating pathway in an in vivo-like model system, and supports future investigations of these agents relevant to cystic fibrosis.
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