Journal
ARCHIVES OF PHARMACAL RESEARCH
Volume 34, Issue 9, Pages 1459-1466Publisher
PHARMACEUTICAL SOC KOREA
DOI: 10.1007/s12272-011-0907-4
Keywords
Anti-inflammatory activity; Antioxidative activity; Cyclooxygenase-2 (COX-2); HaCaT human keratinocyte cells; Inducible nitric oxide synthase (iNOS); Ulmus macrocarpa
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Funding
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0022929]
- National Research Foundation of Korea [2010-0022929] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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The roots of Ulmus macrocarpa Hance (Ulmaceae) have been used as an oriental traditional medicine for the treatment of inflammation, ulcers, cancers, and parasites. Activity guided isolation from the roots of U. macrocarpa yielded three flavonoids [ catechin 7-O-beta-D-apiofuranoside (1), (+)-catechin (2), taxifolin 6-C-glucopyranoside (3)], and one coumarin [ fraxin (4)]. To investigate the antioxidative and anti-inflammatory effects of these compounds, DPPH radical scavenging activity and inhibitory activity against nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells were examined and the expression of inducible NO synthase (iNOS) and cyclooxidase-2 (COX-2) were measured by RT-PCR and Western Blotting in HaCaT cells. Compounds 1, 2, and 3 showed moderate antioxidative activities compared with L-ascorbic acid as a positive control. NO production was reduced and the expressions of iNOS and COX-2 and their mRNA were inhibited by the addition of samples (1-4). These results suggest that the phenolic compounds from the roots of U. macrocarpa might be developed as antioxidant and anti-inflammatory agents.
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