Journal
ARCHIVES OF PHARMACAL RESEARCH
Volume 32, Issue 6, Pages 831-840Publisher
PHARMACEUTICAL SOC KOREA
DOI: 10.1007/s12272-009-1603-5
Keywords
Aralia elata; In vitro anti-inflammatory activity; High-speed counter-current chromatography; Tandem mass spectrometry; Triterpenoid saponin; Elatoside F
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Funding
- Korean Research Foundation [KRF-2007-E0016]
- Ministry of Education, Science and Technology, Republic of Korea
- Korean government [KRF-2008-532-E00032]
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One-step isolation of a saponin from Aralia elata was undertaken using high-speed countercurrent chromatography coupled with evaporative light scattering detection. A triterpenoid saponin, elatoside F, was purified with 96.8% purity using a two-phase-system comprising chloroform-methanol-water-isopropanol. The yield was 35.0 mg from 348.2 mg of the enriched saponin fraction. In vitro anti-inflammatory study demonstrated that elatoside F inhibited lipopolysaccharide-induced nitric oxide production, as well as nuclear factor kappa B activation, in a dose-dependent manner. Two types of mass ionization technique were compared on elatoside F to investigate characteristic fragmentation patterns. MALDI-TOF tandem mass spectrometric fragmentation patterns of sodiated ions provided structural information on glycosidic cleavages and on extensive cross-ring cleavages. Electrospray ionization multiple-stage tandem mass fragmentation of both sodiated and lithiated ions could provide information on glycosidic cleavages. All observed tandem mass fragmentation spectra provided valuable elatoside F structural information when unknown samples from crude extracts are under screening by mass spectrometry.
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