Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 67, Issue 9, Pages 2026-2029Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.67.2026
Keywords
phenol; degradation; tolerance; meta-pathway
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New phenol degrading bacteria with high biodegradation activity and high tolerance were isolated as Burkholderia cepacia PW3 and Pseudomonas aeruginosa AT2. Both isolates could grow aerobically on phenol as a sole carbon source even at 3 g/l. The whole-cell kinetic properties for phenol degradation by strains PW3 and AT2 showed a V-max of 0.321 and 0.253 mg/l/min/(mg protein), respectively. The metabolic pathways for phenol biodegradation in both strains were assigned to the meta-cleavage activity of catechol 2,3-dioxygenase.
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