Journal
ARCHIVES OF PHARMACAL RESEARCH
Volume 31, Issue 10, Pages 1339-1345Publisher
PHARMACEUTICAL SOC KOREA
DOI: 10.1007/s12272-001-2115-0
Keywords
MRSA; Nargenicin; Nocardia sp CS682; Antibiotic; Structure study; Biological activity
Categories
Funding
- Technology Development Program for Agriculture and Forestry
- Ministry of Agriculture and Forestry
- Republic of Korea
Ask authors/readers for more resources
Culture broth of an actinomycete isolate, Nocardia sp. CS682 showed specifically higher antibacterial activity against methicilin resistant Staphylococcus aureus (MRSA). Purified substance from the organism, CS-682, which is active against MRSA and Micrococcus leuteus, is a C28H37NO8 (M+H+, observed: 516.83) and identified as an unusual macrolide antibiotic, nargenicin. The chemical structure of CS-682 was identified by FT-IR, H-1-NMR, C-13-NMR, and (H-1-H-1 and H-1-H-13) COSY. The anti-MRSA activity of CS-682 was stronger than that of oxacillin, vancomycin, monensin, erythromycin, and spiramycin. Phylogenetic analysis showed that strain CS682 is closely related to Nocardia tenerifensis DSM 44704(T) (98.7% sequence similarity), followed by N. brasiliensis ATCC 19296(T) (98.4% sequence similarity). The ability of Nocardia sp. CS682 to produce nargenicin was unique.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available