Journal
DEVELOPMENTAL DYNAMICS
Volume 228, Issue 1, Pages 143-148Publisher
WILEY
DOI: 10.1002/dvdy.10359
Keywords
NG2 proteoglycan; endochondral ossification; intramembranous ossification; tenascin C; alkaline phosphatase; hypertrophic chondrocyte; osteoblast; periosteum; primary ossification
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Funding
- NCI NIH HHS [R01 CA95287] Funding Source: Medline
- NIAMS NIH HHS [R01 AR44400] Funding Source: Medline
- NICHD NIH HHS [P01 HD25938] Funding Source: Medline
- NINDS NIH HHS [R01 NS32717.L] Funding Source: Medline
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We have used immunohistochemistry to study the distribution of the NG2 proteoglycan during bone development in the mouse. At embryonic day 15.5, NG2 was strongly detected in the immature cartilage of developing limbs. After transient down-regulation in mature chondrocytes, NG2 was up-regulated during primary ossification, colocalizing with alkaline phosphatase and tenascin C. In the epiphyseal growth plates of newborn mouse tibia, NG2 and alkaline phosphatase exhibited overlapping patterns of expression by hypertrophic chondrocytes and by osteoblasts surrounding newly formed bone trabeculae. NG2 was down-regulated after puberty, being only faintly detectable in the tibial growth plates of 3-month-old mice. In cranial sutures, NG2 was strongly labeled in osteogenic bone fronts and in the suture matrix. Our results indicate that NG2 expression is up-regulated during both endochondral and intramembranous ossification, but is down-regulated as ossification is completed. Developmental Dynamics 228: 143-148, 2003. (C) 2003 Wiley-Liss, Inc.
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