Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 59, Issue 11, Pages 2611-2617Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S1386-1425(03)00015-5
Keywords
fluorescence; photostability; indocyanine green; gold colloids
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Funding
- NCRR NIH HHS [P41 RR008119, P41 RR008119-13, P41 RR008119-14, RR-08119] Funding Source: Medline
- NIBIB NIH HHS [R01 EB000682-05, EB-00682, R01 EB000682, R01 EB000682-04] Funding Source: Medline
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Photobleaching of fluorophores frequently limits their delectability or observation time. We examined Indocyanine green (ICG) which is widely used in medical testing and is highly unstable. We showed that spatial localization of ICG near metallic gold colloids resulted in increased photostability. This suggests the use of fluorophore-metal conjugates in situations adversely affected by photobleaching. (C) 2003 Elsevier B.V. All rights reserved.
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