Journal
JOURNAL OF BACTERIOLOGY
Volume 185, Issue 17, Pages 5320-5323Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.185.17.5320-5323.2003
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Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.
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