Journal
PLANT SCIENCE
Volume 165, Issue 3, Pages 531-539Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/S0168-9452(03)00212-7
Keywords
molecular marker; simple sequence repeats; Trifolium repens; genetic map; linkage group; DNA sequence analysis
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A framework genetic map of white clover (Trifolium repens L.) has been constructed using an F(2) progeny set derived from the intercross of fourth and Fifth generation inbred genotypes carrying a self-fertile mutation (S(r)). White clover SSR (TRSSR) and AFLP markers were used to derive a map with 135 markers (78 TRSSR loci and 57 AFLP loci) assigned to 18 linkage groups (LGs) with a total map length of 825 cm. Sixteen of these LGs are presumed to correspond to the 16 chromosomes of the white clover karyotype. A limited number of multiple loci were detected. Substantial segregation distortion was observed for both mapped and unmapped loci, with a bias towards heterozygous types and a preponderance of distorted markers on certain LGs. This observation, along with a high degree of residual heterozygosity within the inbred parental genotypes, suggests that reduced individual fitness due to loss of heterosis is a major effect for white clover and will limit the applicability of F2 or backcross mapping strategies for this species. A core set of map-assigned co-dominant, single locus SSR markers has been defined for whole genome scans of genetic variability in white clover. Crown Copyright (C) 2003 Published by Elsevier Ireland Ltd. All rights reserved.
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