Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 17, Pages 1445-1449Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1025059713425
Keywords
Bacillus circulans; B. megaterium; B. subtilis; endo-beta-1,3-1,4-glucanase; shuttle plasmid
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A gene coding for endo-beta-1,3-1,4-glucanase (lichenase) containing a recombinant plasmid, pLL200K, was transferred from Bacillus circulans into a new shuttle plasmid, pLLS920, by ligating linearized DNAs of pLL200K and pUB110. B. subtilis RM125 and B. megaterium ATCC14945 transformed with pLLS920 produced the endo-beta-1,3-1,4-glucanase. The enzyme was produced during active growth with maximum activity. The B. subtilis (pLLS920) enzyme was 83 times (8522 mU ml(-1)) more active than that of the gene donor cells (103 mU ml(-1)). The B. megaterium (pLLS920) enzyme was 7 times (735 mU ml(-1)) more active than that of the gene donor cells. While E. coli secreted only about 10% of the produced enzyme, B. subtilis excreted the enzyme completely into the medium and B. megaterium by about 98%. The plasmid pLLS920 was stable in B. megaterium (98%), and in B. subtilis (51%) but not in E. coli (29%).
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