Journal
BIOTECHNOLOGY LETTERS
Volume 25, Issue 18, Pages 1509-1514Publisher
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1025438701383
Keywords
electroporation; glyceraldehyde-3-phosphate dehydrogenase promoter; green fluorescent protein; hygromycin B resistance; Monascus purpureus
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Monascus purpureus was transformed into hygromycin B resistance with hygromycin B phosphotransferase (hph) fused to Aspergillus nidulans trpC or a putative Monascus purpureus gpd1 promoter by electroporation. Among five strains, only M. purpureus DSM1397 was a competent recipient. Normal growth and sporulation on media containing up to 500 mg hygromycin B l(-1) occurred up to five generations. Upon transformation of the strain with the green fluorescent protein gene (sgfp) as a model gene and hph as a selection marker, characteristic green fluorescence was observed under fluoromicroscopy indicating successful transformation.
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