Journal
BIOPHYSICAL CHEMISTRY
Volume 105, Issue 2-3, Pages 681-699Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0301-4622(03)00097-8
Keywords
triplex stabilization; Hofmeister anion series
Funding
- NIGMS NIH HHS [GM42936, GM08309] Funding Source: Medline
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The thermal stability of the triplexes d(C+ -T)(6):d(A-G)(6).d(C-T)(6) and d(T)(21).:d(A)(21).d(T)(21) was studied in the presence of high concentrations of the anions Cl-, HPO4-, CH3COO-, SO42- and ClO4-. Thermally-induced triplex and duplex transitions were identified by UV- and CD-spectroscopy and T-m values were determined from melting profiles. A thermodynamic analysis of triplex transitions shows the limitations of commonly used treatments for determining the associated release or uptake of salt, solute or water. Enhancement of the stability of these triplexes follows the rank order of the Hofmeister series for anions of sodium and ammonium salts, whereas water structure-breaking solutes have the opposite effect. The rank order for the Hofmeister series ClO4- < I- < Br- < Cl- < HPO42- < SO42- is shown to follow their effective surface charge densities. (C) 2003 Elsevier Science B.V. All rights reserved.
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