Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 142, Issue 1-2, Pages 58-66Publisher
ELSEVIER
DOI: 10.1016/S0165-5728(03)00261-3
Keywords
myotube; terminal complement complex c5b-9; c-fos; serum response factor; ERKI
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Funding
- NINDS NIH HHS [R0-1 NS199006, NS42011] Funding Source: Medline
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Sublytic C5b-9 alters the molecular phenotype of myotubes by inhibiting muscle-specific gene expression. Here, we showed that C5b-9 induced c-fos mRNA and transcription. Using c-fos promoter-CAT constructs and electrophoretic mobility shift assay (EMSA), the minimal c-fos promoter activity was shown to increase within 30-min exposure to serum C5b-9, which also induced the binding of serum response factor (SRF), along with ternary complex factor (TCF) Elk1 and Sap1a to the serum response element. C5b-9 activated ERK1, which in turn activated Elk1 in myotubes. We propose that c-fos gene transcription associated with myotube dedifferentiation is induced by C5b-9 through ERK1-mediated assembly of serum response factor-ternary complex. (C) 2003 Elsevier B.V. All rights reserved.
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