Journal
BIOCHEMICAL JOURNAL
Volume 374, Issue -, Pages 497-503Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BJ20030452
Keywords
BRCA2-associated factor 35 (BRAF35); human kinesin superfamily protein member 4 (KIF4); protein-protein interaction; yeast two-hybrid analysis
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A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.
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