Journal
PEPTIDES
Volume 24, Issue 9, Pages 1303-1313Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2003.07.013
Keywords
HIV-1 gp41; HIV-1fusion inhibitors; native PAGE; fluorescence; HPLC
Funding
- NIAID NIH HHS [AI 46221] Funding Source: Medline
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Triggered by receptor binding of gp120, the human immunodeficiency virus type 1 (HIV-1) gp41 changes its conformation to a fusogenic six-helix bundle structure. In the present study, this core conformation modeled by the peptides derived from the gp41 N- and C-terminal heptad repeat regions was determined by fluorescence native polyacrylamide gel electrophoresis and size exclusion high-performance liquid chromatography (HPLC). Two previously described small molecule HIV-1 fusion inhibitors significantly blocked the six-helix bundle formation. It suggests that these biophysical techniques can be used in a novel way to study the conformational change of gp41 during virus entry into cells and to identify HIV-1 fusion inhibitors. (C) 2003 Elsevier Inc. All rights reserved.
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