Journal
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Volume 377, Issue 2, Pages 346-349Publisher
SPRINGER-VERLAG BERLIN
DOI: 10.1007/s00216-003-2098-4
Keywords
fluorescent technique; perylene colloids; cetyltrimethylammonium bromide (CTAB); DNA
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Fluorescent perylene colloids in the 80-90 nm size range have been prepared by the reprecipitation method. These nanoparticles were modified by cetyltrimethylammonium bromide (CTAB) which inhibited their growth. The nanoparticles also readily interacted with DNA. The fluorescence emission was measured at lambda(ex)/lambda(em)=400/565 nm. The fluorescence decrease of colloid-CTAB in aqueous solution was measured in the presence of nucleic acids. Under the optimum conditions, the ratio of fluorescence intensity in the absence and presence of nucleic acids was proportional to the concentration of nucleic acids over the range 0.02-5.1 mug mL(-1) for FS (fish sperm) DNA or CT (calf thymus) DNA. The detection limits were 0.01 mug mL(-1) for FS DNA and 0.012 mug mL(-1) for CT DNA, respectively. Based on this approach, a new quantitative method for DNA assay is presented in this paper.
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