Journal
JOURNAL OF IMMUNOLOGY
Volume 171, Issue 5, Pages 2183-2186Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.171.5.2183
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Funding
- NIAID NIH HHS [5R01 AI 022033] Funding Source: Medline
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We quantitated the amounts of peptides from hen ea white lysozyme presented by I-A(k) molecules in APC lines. The large chemical gradient of presentation of the four hen egg-white lysozyme epitopes observed in cell lines expressing HLA-DM or H-2DM (referred to in this study as DM) was significantly diminished in the T2.A(k) line lacking DM. Differences in levels of presentation between wild-type and DM-deficient APC were observed for all four epitopes, but differences were most evident for the highest affinity epitope. As a result of these quantitative differences in display, presentation of all four epitopes to T cells was impaired in the line lacking DM. The binding affinity of the pool of naturally processed peptides from DIM-expressing lines was higher than that from the DM-deficient line. Thus, using a direct biochemical approach in APC, we demonstrate that DM influences the selection of peptides bound to MHC class II by favoring high affinity peptides.
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