Rhodococcus tukisamuensis sp nov., isolated from soil

A novel strictly aerobic, heterotrophic, mesophilic bacterium, strain Mb8(T), was isolated from soil in Sapporo City, Hokkaido, Japan. The G + C content of strain Mb8T was 66.0 mol%. It had mycolic acids with 44-52 carbon atoms and C16:0 and C18:1(9) as the major fatty acids. The major isoprenoid quinone was MK-8(H-2). The cell wall contained meso-diaminopimelic acid, arabinose and galactose. 16S rDNA, chemotaxonomic and morphological data indicated that this strain clearly belonged to the genus Rhodococcus. Based on phenotypic properties and DNA-DNA hybridization data, strain Mb8(T) (=JCM 11308 (T) NCIMB 13903(T)) has been assigned to the genus Rhodococcus as the type strain of Rhodococcus tukisamuensis sp. nov.