Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 50, Issue 3, Pages 493-499Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/mrm.10565
Keywords
water exchange; brain; magnetic resonance; membrane permeability; cell water content
Funding
- NCI NIH HHS [CA83060] Funding Source: Medline
- NIGMS NIH HHS [GM32125] Funding Source: Medline
- NINDS NIH HHS [NS40801, NS35912] Funding Source: Medline
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This report describes the measurement of water preexchange lifetimes and intra/extracellular content in intact, functioning mammalian brain. Intra- and extracellular water magnetic resonance (MR) signals from rat brain in vivo were quantitatively resolved in the longitudinal relaxation domain following a ministration of an MR relaxation agent into the extracellular space. The estimated intracellular water content fraction was 81% +/- 8%, and the intra- to extracellular exchange rate constant was 1.81 +/- 0.89 s(-1) (mean +/- SD, N = 9), corresponding to an intracellular water preexchange lifetime of similar to550 ms. These results provide a temporal framework for anticipating the water exchange regime (fast, intermediate, or slow) underlying a variety of compartment-sensitive measurements. The method also supplies a means by which to evaluate membrane water permeability and intra/extracellular water content serially in intact tissue. The data are obtained in an imaging mode that permits detection of regional variations in these parameters. (C) 2003 Wiley-Liss, Inc.
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