Journal
MOLECULAR ECOLOGY NOTES
Volume 3, Issue 3, Pages 406-408Publisher
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1471-8286.2003.00466.x
Keywords
China; microsatellites; Philippines; Schistosoma japonicum
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The ability of microsatellite loci to reveal genetic diversity within the trematode Schistosoma japonicum is demonstrated. Eleven novel microsatellite markers were isolated, assessing variability within 80 S. japonicum individuals from three Chinese and one Philippine population. Novel primers were also tested upon S. mansoni and S. haematobium. Eight primers showed polymorphic amplification from all S. japonicum groups, three amplified S. mansoni DNA, but none amplified S. haematobium DNA. Only six of 27 previously isolated S. mansoni-specific primers amplified S. japonicum DNA. Allelic diversity and observed heterozygosity ranged from 4 to 21 and 0.05 to 0.82, respectively, suggesting high variability.
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