Journal
ARCHIVES OF ORAL BIOLOGY
Volume 57, Issue 4, Pages 364-368Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2011.09.005
Keywords
Povidone-iodine; Periodontopathic bacteria; Biofilm; Antibacterial agent; P. gingivalis; F. nucleatum
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Funding
- Japan Society for Promotion of Science [C-22592317, C-17592164]
- Grants-in-Aid for Scientific Research [24592965, 22592317, 21592344, 23792381, 21592501] Funding Source: KAKEN
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Objective: To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. Design: Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated. Results: In the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration. Conclusion: These results suggest that 30 s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm. (C) 2011 Elsevier Ltd. All rights reserved.
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