Journal
ARCHIVES OF ORAL BIOLOGY
Volume 57, Issue 12, Pages 1668-1675Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2012.04.022
Keywords
Neural crest cells; Neural crest marker; Periodontal ligament
Categories
Funding
- Japan Society for the Promotion of Science
- NIDCR [K99DE021054, R01DE020843, DE019527, K08DE018695]
- Intramural Research Program of the NIEHS/NIH [ES071003-11]
- [21890081]
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Objective: Cells derived from the neural crest (NC) contribute to the development of several adult tissues, including tooth and periodontal tissue. Here, two transgenic lines, Wnt1-Cre/ZEG and PO-Cre/ZEG, were analysed to determine the fate and distribution of neural crest cells (NCCs) in adult mouse periodontal ligament (PDL). Design: Paraffin-embedded and decalcified histology samples were prepared from Wnt1-Cre/ZEG and PO-Cre/ZEG mice that were 4-, 8-, or 12-weeks old. Expression of GFP (NC-derived cells), NC-markers (Slug, AP-2 alpha, HNK-1, p75NTR and Nestin), and mesenchymal stem cell markers (CD29 and STRO-1) were examined using immunohistochemistry. Results: In four-week-old Wnt1-Cre/ZEG mice, GFP((+)) NC-derived cells were specifically detected in the mid-zone of PDL. The GFP((+)) cells constituted 1.4% of all cells in PDL, and this percentage decreased as the mice aged. The distribution and prevalence of GFP((+)) cells were comparable between Wnt1-Cre/ZEG and PO-Cre/ZEG mice. NC-marker ((+)) cells were expressed only in GFP((+)) cells while MSC markers were detected only in GFP((-)) cells. Conclusion: The prevalence and specific distribution of NC-derived cells in adult PDL of Wnt1-Cre/ZEG and PO-Cre/ZEG mouse were examined. Interestingly, various NC markers, including markers for undifferentiated NCCs, were still expressed at high levels in GFP((+)) cells. These observations may indicate that labelled cells in the Wnt1-Cre/ZEG and PO-Cre/ZEG mice did not constituted all NC-derived cells, but rather an interesting subset of NC-derived cells. These findings may be useful in understanding the homeostatic character of the PDL and contribute to establishing successful periodontal tissue maintenance. (C) 2012 Elsevier Ltd. All rights reserved.
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