Journal
ARCHIVES OF ORAL BIOLOGY
Volume 55, Issue 9, Pages 648-654Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2010.06.005
Keywords
Human; Dental pulp cells; Hypoxia; Proliferation; Differentiation
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Funding
- Ministry of Education, Science, and Culture of Japan [20890091]
- Grants-in-Aid for Scientific Research [20890091] Funding Source: KAKEN
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The hypoxia condition was expected to be suitable for the establishment and maintenance of human dental pulp cells (hDPCs), because they reside in a low-oxygen environment in vivo. Therefore, we presently examined the effects of hypoxia on the proliferation and differentiation of hDPCs in vitro. hDPCs grown under 3% O-2 showed a significantly higher proliferation rate than those under 21% O-2. Then, we prepared hypoxic cultures of hDPCs from older patients' teeth having inflammation and succeeded in recovering and expanding a small number of hDPCs. These cells were confirmed to have capability for osteo/odontogenic differentiation. Hypoxia suppressed the osteo/odontogenic differentiation of hDPCs in vitro and increased the number of cells expressing STRO-1, an early mesenchymal stem cell marker. This simple method will increase the possibility to obtain living hDPCs from damaged and/or aged tissues, from which it is ordinarily difficult to isolate living stem cells with differentiation capability. (C) 2010 Elsevier Ltd. All rights reserved.
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