Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 31, Issue 1-4, Pages 159-168Publisher
ELSEVIER
DOI: 10.1016/S0927-7765(03)00135-8
Keywords
visualisation; biopolymer mixtures; confocal scanning laser microscopy; covalent labelling
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Confocal scanning laser microscopy (CSLM) has been used to study the behaviour of mixtures of proteins, gelatine, whey proteins and beta-lactoglobulin, and polysaccharides, dextran, gellan gum, carrageenan, gum Arabic, and starch. CSLM proved to be a suitable technique to visualise the microstructure of these (phase separated) mixtures in two and three-dimensional images. Contrast through fluorescence is obtained either by covalent labelling (polysaccharides and proteins) or non-covalent labelling (proteins and starch). Double and triple labelling allows the visualisation of individual components in a complex mixture of biopolymers. (C) 2003 Elsevier B.V. All rights reserved.
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