Journal
PLANT CELL REPORTS
Volume 22, Issue 2, Pages 129-134Publisher
SPRINGER-VERLAG
DOI: 10.1007/s00299-003-0657-y
Keywords
gene silencing; promoter; Oryza sativa; transgenic plant; ubiquitin
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Strong constitutive promoters form a cornerstone for basic and applied research using transgenic plants. GUS (beta-glucuronidase) expression levels from constructs containing RUBQ1 or RUB2 rice ubiquitin promoters were 8- to 35-fold higher in transgenic rice [Oryza sativa (L.)] plants, respectively, when compared to the 35S promoter. Deletion analysis of the 5'-upstream region of RUBQ2 revealed a putative enhancer region that produced a 2.4-fold increase in transient GUS expression. Southern blot analysis showed that three to seven copies of the GUS gene were stably inserted into R0 and R1 plants and inherited in a monogenic fashion.
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