Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1011, Issue 1-2, Pages 181-192Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)01181-6
Keywords
interfaces; CE-MS; chip technology; peptides
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A robust and simple interface for microchip electrophoresis-mass spectrometry (MCE-MS) was developed using a spray nozzle connected to the exit of the separation channel of the microchip. The spray nozzle was attached to the microchip using a polyether ether ketone screw without adhesive, thus allowing easy replaced. Sample injection and electrophoretic separation was performed by control of the voltage only. The analysis of a few basic drugs was performed using the optimized MCE-MS system. The separation was improved by using a high-viscosity separation buffer and a spray nozzle with a small bore size. This system was also applied to the separation of peptides and protein-trypsin digests. Sample adsorption was minimized by adding acetonitrile to the separation buffer when using a quartz microchip. (C) 2003 Elsevier B.V. All rights reserved.
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