Journal
JOURNAL OF CELL BIOLOGY
Volume 162, Issue 6, Pages 1079-1088Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303023
Keywords
actin; lamella; polymerization; SCAR; cytokinesis
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Funding
- NIGMS NIH HHS [F32 GM064966, R01 GM038499, 5F32GM064966-02] Funding Source: Medline
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Cell migration occurs through the protrusion of the actin-enriched lamella. Here, we investigated the effects of RNAi depletion of similar to90 proteins implicated in actin function on lamella formation in Drosophila S2 cells. Similar to in vitro reconstitution studies of actin-based Listeria movement, we find that lamellae formation requires a relatively small set of proteins that participate in actin nucleation (Arp2/3 and SCAR), barbed end capping (capping protein), filament depolymerization (cofilin and Aip1), and actin monomer binding (profilin and cyclase-associated protein). Lamellae are initiated by parallel and partially redundant signaling pathways involving Rac GTPases and the adaptor protein Nck, which stimulate SCAR, an Arp2/3 activator. We also show that RNAi of three proteins (kette, Abi, and Sra-1) known to copurify with and inhibit SCAR in vitro leads to SCAR degradation, revealing a novel function of this protein complex in SCAR stability. Our results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells.
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