Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 83, Issue 6, Pages 743-747Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/bit.10707
Keywords
beta-cyclodextrin; dextran; enzyme stability; trypsin
Categories
Ask authors/readers for more resources
Dextran modified with the mono-6-pentylene-diamino-6-deoxy-beta-cyclodextrin derivative was evaluated as a thermoprotectant additive for trypsin. The optimum temperature for trypsin activity was increased by 7degreesC in the presence of this polymer. The enzyme thermo-stability was increased from 48.5 to 64degreesC over 10 min of incubation, and the activation free energy of thermoinactivation at 50degreesC was increased by 4.1 kJ/mol in the presence of the additive. Trypsin was 6-fold more resistant to autolytic inactivation at alkaline pH in the presence of the polymer. (C) 2003 Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available