Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1013, Issue 1-2, Pages 111-122Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)01359-1
Keywords
capillary electrophoresis; peak shaped; polydimethylsiloxane
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Several anomalies, e.g., in peak shape, migration time, and baseline drift, all due to pressure-driven backflow, were previously reported to occur during serial injection on capillary electrophoresis (CE) chips. Since these anomalies were worse for polydimethylsiloxane (PDMS) microchips than for glass microchips, reproducible data on PDMS microchips were difficult to obtain. In this paper, we found that these problems were affected by the hydrophilic or hydrophobic properties of the reservoirs on the microchip and demonstrated that these anomalies were reduced by converting the hydrophobic properties of the reservoirs on the PDMS microchip into hydrophilic ones. Thus, compared with hydrophobic reservoirs, hydrophilic reservoirs were suitable for the formation of a stable plug. Several chip designs were suggested to reduce these pressure-driven backflows. (C) 2003 Elsevier B.V. All rights reserved.
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