Journal
TALANTA
Volume 61, Issue 1, Pages 43-51Publisher
ELSEVIER
DOI: 10.1016/S0039-9140(03)00358-8
Keywords
nitric oxide; T-HUVEC; pyrrole; porphyrins
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Pyrrole-functionalised tetracarboxyphenyl porphyrin and trimethoxyphenylcarboxy-phenyl porphyrin containing Ni, Mn and Pd as the central metal ion were used to modify Pt-disk microelectrodes (null set 50 mum) (by repetitive cyclic voltammetry, dip-dry and pulse-amperometry methods) for the detection of nitric oxide (NO). Electrodes modified with Mn(II) trimethoxyphenylcarboxyphenyl porphyrin using the pulse amperomery approach, were found to be sensitive, stable and fast in response towards the oxidation of NO. Thus, they were used for the detection of NO release from a population of transformed human umbilical vein endothelial cells (T-HUVEC) into a droplet of electrolyte solution following stimulation with vascular endothelial growth factor (VEGF). The electrode surface was covered with an additional layer of Nafion to prevent interference from anionic molecules such as nitrite. (C) 2003 Elsevier B.V. All rights reserved.
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