Journal
PEPTIDES
Volume 24, Issue 10, Pages 1493-1500Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2003.10.006
Keywords
allatostatins; endocrinology; juvenile hormone; Locusta migratoria; MALDI-TOF; mass spectrometry; neuropeptides; peptidome; pyrokinins; Schistocerca gregaria
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The peptidomes of the corpora allata of Locusta migratoria and Schistocerca gregaria were investigated by both matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nanoscale liquid chromatography quadrupole time-of-flight tandem mass spectrometry (nanoLC-Q-TOF MSMS). The pyrokinin (-FXPRLamide) family seems to be predominant. In addition to the known pyrokinins, we de novo sequenced four pyrokinins in L migratoria and five in S. gregaria. In addition, one pyrokinin-like peptide (-PRLamide) was identified in S. gregaria. Besides the -(FX)PRLamides, FLRFamide-l, the allatostatins (A family) and numerous as yet unidentified peptides are also present in the corpora allata. (C) 2003 Elsevier Inc. All rights reserved.
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