Journal
JOURNAL OF SEPARATION SCIENCE
Volume 26, Issue 15-16, Pages 1451-1454Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200301512
Keywords
aptamers; liquid chromatography; affinity chromatography; molecular recognition
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Aptamers are oligonucleotides which are isolated in vitro based on their affinity for a target molecule. These molecules hold promise for use in analytical methods which rely on molecular recognition. In this study, we report the immobilization of an RNA aptamer on the inner walls of fused silica capillaries. Due to the small size of these molecules, the resulting surface coverage of these capillaries made them suitable for use in open tubular liquid chromatographic studies. These capillaries also exhibited stability when an organic modifier was added to the mobile phase. These characteristics of aptamers make them desirable over traditional affinity chromatography stationary phases.
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