Journal
CARBOHYDRATE POLYMERS
Volume 54, Issue 1, Pages 13-19Publisher
ELSEVIER SCI LTD
DOI: 10.1016/S0144-8617(03)00149-8
Keywords
Sophora subprostrata; Leguminosae; roots; polysaccharide
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The crude polysaccharide SSa, obtained from the roots of Sophora subprostrata by boiling-water extraction and ethanol precipitation, was fractionated by anion-exchange and gel permeation chromatography, giving four polysaccharide fractions termed SSa1, SSa2, SSa3, and SSa4. Their structural features were investigated by sugar analysis, methylation analysis, partial hydrolysis, and NMR spectroscopy. SSa1 and SSa2 were both characterized as highly branched amylopectins with small average unit chain length of 8-10 except that SSa2 was contaminated with small amount of arabinogalactan. SSa3 was mainly an arabinogalactan, attached to a rhamnogalacturonan core. SSa4 was a rhamnogalacturonan I (RG-I) pectic polysaccharide having a backbone consisting of 1,4-linked alpha-D-galacturonic acid and 1,2-linked alpha-L-rhamnose, with various branches attached to O-4 of L-rhamnose. (C) 2003 Elsevier Ltd. All rights reserved.
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